Nitrotyrosine ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the competitive measurement of Free Nitrotyrosine protein in plasma, serum, cell lysates, urine, and other sample matrices. The ELISA utilizes an Nitrotyrosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 62.5 to 8000 nM Free Nitrotyrosine, with a sensitivity of 50 nM. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.