Each of these modular, animated, narrated eLearning courses were developed to provide a succinct, contextual summary of common laboratory techniques and methods used by researchers with an interest in protein and cell analysis workflows. At the end of each course, take an opportunity to apply the theory that was provided by completing the associated practical application exercises. We then invite you to download the eLearning certificate of completion for each course that you master.

If you have already registered for the Protein and Cell Analysis Education platform, you simply need to click on the “Start the course” link for the course you are interested in and log in with your credentials.

If you have not registered for the Protein and Cell Analysis Education platform or are not a LabRoots member already, you will need to first sign up by clicking the SIGN UP button. Once you have completed that form, you can click on the “Start the course” link and sign in.


eLearning course

Topics covered

T Cell Stimulation and Proliferation




Part 1: T cell biology in translational biomedical science; basic T cell biology in the context of the wider immune system; T cell structure and function; T cell receptor signaling; T cell activation and proliferation (antigen presenting cell interactions and T effector functions; T cell subsets (including subset-specific signature proteins)

Part 2:  T cell isolation and in vitro activation; assessment of T cell activation, proliferation, and differentiation by these methods:

  • Flow cytometric analysis
  • Enzyme-linked immunosorbent assays
  • Multiplex Luminex™ assays


Practical Application —Flow Cytometry


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eLearning course

Topics covered

Protein Sample Preparation: Techniques and Applications









Part 1: Total protein extraction; cell disruption methods: (reagent-based and physical); subcellular fractionation; sample preparation for downstream assays such as:

  • Western blotting
  •  ELISA
  • Immunoprecipitation
  • Mass spectrometry


Practical Application—Part 1: Protein Isolation

Part 2:  Hydrophobic interaction chromatography (HIC); size exclusion chromatography (SEC); ion exchange chromatography (IEX); affinity chromatography (AC); protein cleanup methods:

  • Desalting
  • Buffer exchange
  • Dialysis


Practical Application—Part 2: Protein Purification


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For research use only. Not for use in diagnostic procedures.