To really see what’s going on with your AAV’s capsid, you’ve got to keep an eye out for aggregation, protein unfolding, and even leaking DNA. One-trick stability assays that only look for protein unfolding won’t track DNA uncoating, and the impact of a new buffer or capsid mutation can be missed. Uncle sees what’s going on for both proteins and DNA, so you can quickly measure how your whole AAV reacts to a lower pH, an excipient, a different buffer, or an elevated temperature. This seminar will cover how to quantify genome ejection, aggregation and capsid stability behavior for several AAV serotypes and give examples of when buffers really change the story of your AAV’s stability.

 

Learning Objectives