NOV 20, 2019 8:00 AM PST

Approaches to nucleic acid isolation: from sample to NGS analysis

Sponsored by: Cytiva
C.E. Credits: P.A.C.E. CE Florida CE
  • NGS Core Facility Manager, Private Molecular Diagnostic Lab, Cologne, Germany.
      Dr Stefanie Stepanow is an NGS core facility manager in a private molecular diagnostic lab in Cologne, Germany. She is responsible for the establishment and validation of new NGS assays as well as monitoring and optimization of current NGS workflows. For almost 9 years, Stefanie has worked routinely with different NGS techniques (Illumina™, Ion Torrent™) and a wide range of sample materials while gaining experience in optimizing NGS workflows to achieve meaningful results from difficult materials. Previously, she worked as a scientist in an NGS core facility with focus on clinical and biological research in Duesseldorf, where she established and standardized the NGS techniques and attained lab accreditation.

    DATE:   November 20, 2019
    TIME:   8:00am PST, 11:00 EST, 4:00pm GMT, 5:00pm CET
    Even as next-generation sequencing (NGS) has become widely used, and the costs have been reduced, it remains critical to achieve reliable and robust data at the end of the sequencing run. Sequencing labs have very little control over the quantity and quality of the sample received from the pathology lab or clinic, yet sample quality can have a major impact on the downstream processes.
    While the manual, column-based approach to nucleic acid isolation is still widely used, scientists under pressure from high-throughput demands are turning their attention to superparamagnetic beads. These are automation friendly and well-suited for a range of applications, including sample preparation for next generation sequencing (NGS). Each technique has pros and cons, and selection will depend on sample type, throughput requirements, targets, and other factors.
    Join Dr. rer. nat. Stefanie Stepanow on November 20, 2019 for a free webinar reviewing the column and bead-based approaches to nucleic acid isolation for DNA in molecular diagnostic applications and optimization of the process from sample assessment to NGS analysis.
    In this webinar you will:
    • Demonstrate how to check sample quality against set criteria and assess the potential impact on data outcomes in relation to genomic and prenatal testing applications
    • Review modifications to workflows, protocol steps and NGS analysis to manage the quality of complex samples such as FFPE and liquid biopsy
    • Compare the benefits and drawbacks of different approaches to nucleic acid isolation with particular focus on column-based and magnetic bead-based approaches
    • Review sequencing data comparing column-based and bead-based approaches
    Webinars will be available for unlimited on-demand viewing after live event.
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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