JUN 27, 2019 9:00 AM PDT

CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system for studying cellular microenvironment

Speaker
  • Post Doctoral Associate, Florida International University
    Biography
      Dr. Vivek Kamat received his Ph.D. from the University of Pune (India) in 2018. During his doctoral training under the supervision of Dr. Kishore Paknikar and Dr. Dhananjay Bodas at Agharkar Research Institute (Pune, India), he mastered the size-controlled synthesis of a variety of nanoparticles and the process of soft lithography fabricating complex microstructures using natural and artificial polymers. His work involved the size-controlled synthesis of drug-loaded nanoparticles and assessing their biological effect on cancer cell lines. Further Dr. Kamat worked in the area of tumor microenvironment specifically understanding cellular response and growth in confined volumes. He has experience in modeling and simulating biological system Insilco using COMSOL to study fluid dynamics, particle transport, and heat transfer. Dr.Kamat has experience working in interdisciplinary areas involving Cell biology, Nanotechnology, Electronics, and Microfabrication.

      Dr. Kamat joined the laboratory of Professor Shekhar Bhansali at Florida International University Department of Electrical engineering (FIU, Miami, USA) where he is working on understanding circulating tumor cells using electrical and optical methods to investigate cell-cell interactions and tumor microenvironment. He is also involved in integrating Artificial Intelligence and Machine learning to understand and predict cancer progression. He has active collaboration with Professor Kalai Matee at the Herbert Wertheim College of Medicine (FIU) working on transcriptomics analysis of cells growing in continuous flow conditions in microfluidic devices and along with his research he is involved in active teaching at the undergraduate level at FIU College of Engineering.

    Abstract
    DATE:  June 27, 2019
    TIME:   9:00am PDT, 12:00pm EDT
     
    At present cancer research focuses on three major areas viz. cancer diagnostics, drugs development, and next-generation therapies. About 90% of the in-vitro research rely on traditional two-dimensional (2D) monolayer cell culture systems. 2D cell culture systems fail to accurately recapitulate the structure, function, physiology of living tissues, due to which the various studies such as assessing the efficacy of new drugs, study gene expressions, metabolic pathways, and cell proliferation do not correlate to actual In-vivo scenario. In contrast, a 3D cell culture system promotes many biological relevant functions which are not observed in 2D. The primary reason for this is attributed to two reasons: 1)In the real scenario cancer cells experience limited diffusion of oxygen, nutrients and signaling molecules in a dynamic way, which the 2D fails to mimmick. 2)Cellular interaction, function, growth and signaling all occurs in a highly complex 3D architecture with the influence of extracellular matrix and other regulatory factors, which cannot be recapitulated in 2D systems.

    To achieve this dynamic coordination microfluidic cell culture systems can be employed which can provide a continuous flow of nutrients, exchange of gases and other regulatory factors in a well-controlled manner. Such a system is ideal to mimic the in vivo environment of cells. The idea to couple microfluidics with 3D cell culture system will allow study of cellular functions such as proliferation in dynamic systems, cell-cell interaction and cellular response to the external environment in a much realistic environment. Further microfluidic systems give an opportunity to study cellular interaction by fabricating microstructures and artificial scaffolds to study cellular movements and the underlying mechanobiology. Using the microfluidic approach better drugs and therapies can be developed which can be easily translated to invivo systems and hence can bridge the gap between invitro and invivo system.
     
    Learning Objectives:
    • Understand the difference between conventional and Microfluidic cell culture
    • learn the process of fabricating microfluidic cell culture chips and the requirements to establish a successful cell culture
    • Carrying out biological assay in microfluidic cell culture systems to understand cellular function
     
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.
     

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