Scientist III, Protein Biology, Antibodies and Immunoassays, Thermo Fisher Scientific
BIOGRAPHY
Speaker
Event Date & Time
DATE: October 31, 2018
TIME: 8:00am PT, 10am CT, 11:00am ET
Abstract
Chromatin immunoprecipitation (ChIP) is a technique researchers employ to obtain a snapshot of protein-DNA interactions in the cell. ChIP provides a picture of where your protein of interest is in the genome, and understanding these interactions provides significant insight into gene expression. ChIP involves many steps and can be a challenging experiment. This webinar discusses the major steps in ChIP and provides many tips for a successful ChIP experiment, including things to consider before you start your ChIP experiment, methods for chromatin fragmentation, antibody selection, primer design, and how to analyze your qPCR data. As ChIP often needs to be tailored for cell type and protein of interest, the presenter will share tips for optimization and troubleshooting at each step.
Learning objectives:
- Learn how to design a successful ChIP experiment
- Understand different methods for performing ChIP and how they can impact your experiment