JUN 24, 2020 8:00 AM PDT

CRISPR-Cas assisted genetics in intestinal organoids

Sponsored by: GenScript
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Group Leader at Institute of Molecular Biotechnology (IMBA)
    Biography
      Dr. Bon-Kyoung Koo is an experienced mouse geneticist actively employing the emerging organoid culture technology to study stem cell maintenance and activation in the mouse and human intestine and colon. Following his PhD in the laboratory of Prof. Young-Yun Kong at POSTECH, Dr. Koo joined the group of Prof. Hans Clevers at the Hubrecht Institute, Netherlands in 2009. Here he witnessed the first establishment of the Lgr5 + cell-derived intestinal organoid culture system. In the following years Dr. Koo has utilized this novel culture system to perform sophisticated gene correction with CRISPR-Cas9, to unravel the molecular regulation of adult stem cells and to prove the stem cell properties of a Troy +, quiescent reserve stem cell population in the stomach. After first group leader position at the Cambridge Stem Cell Institute (2013-17), he moved his group to IMBA. Currently, his group focuses on clonal dynamics of adult epithelial stem cells both in homeostasis and during injury mediated repair.

    Abstract

    DATE:  June 24, 2020

    TIME:   8:00am PT

     

    The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D intestinal organoid culture system from mouse and human tissues. This novel culture system faithfully recapitulates various aspects of the intestinal epithelium in vitro with remarkable long-term expansion capacity and genetic stability. Thus, the model is recognized as a suitable in vitro model system for genetic studies. To exploit all the potential of this culture, protocols have been fully optimized for primary establishment, maintenance, cryopreservation, plasmid transfection and viral transduction. A number of examples will be shown to introduce how to apply CRISPR technology and organoid models for genetic studies, including simultaneous paralogue knockout, functional genetic screening and precise gene correction.

     

    Learning Objectives:

    • Discuss adult stem cell-derived 3D organoid system
    • Familiarize yourself with all available genetic tools for the organoid system

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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