OCT 27, 2021 10:00 AM PDT

A CRISPR powered NGS library prep to improve viral, bacterial and host transcript detection in shotgun metagenomic sequencing

Sponsored by: Jumpcode Genomics
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
Date:  October 27, 2021
Time: 10:00am (PDT),  1:00pm (EDT)
 
The COVID-19 pandemic has brought global awareness to the dangers of emerging pathogens to human health and welfare. Common molecular tools used at scale, such as PCR, are best suited for detecting known pathogens. Preparing humanity against future zoonotic pandemics requires unbiased surveillance tools, such as next generation sequencing (NGS). This approach enables the unbiased detection of viral sequences (known and unknown), identifies possible co-infections, and provides insights into host transcriptional response. This webinar will introduce a CRISPR-based strategy improving sequence complexity and discovery in libraries prepared from complex biological samples. The critical component enabling this approach is Jumpcode Genomics’ CRISPRclean technology which removes abundant mammalian and bacterial ribosomal RNA sequences from NGS libraries. We will review data from >40 nasopharyngeal samples collected from SARS-CoV-2 testing, demonstrating >90% depletion of all human and bacterial ribosomal sequences, 3-to-7-fold increase in viral and bacterial transcriptome coverage and 5-to-10-fold increase in coverage of mid to low expressing host transcripts.
 
Learning Objectives
  • Discuss and understand the importance of shotgun sequencing approaches in detection of novel or variant pathogens
  • Discuss importance of depletion of abundant uninformative sequences in improving sequencing coverage
  • Discover how CRISPR specificity can enhance RNA-Seq library preparation
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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