MAY 03, 2017 10:00 AM PDT

What are the current advances in Flow Cytometry?

Sponsored by: Thermo Fisher Scientific
  • SVM Professor of Cytomics, Professor of Biomedical Engineering, Purdue University
      J. Paul Robinson is the SVM Professor of Cytomics in the College of Veterinary Medicine and a professor of biomedical engineering in the Weldon School of Biomedical Engineering at Purdue University. He received his Ph.D. in Immunopathology from the University of New South Wales, Sydney, Australia. He completed a postdoctoral fellowship at the University of Michigan Medical School. He is currently the director of the Purdue University Cytometry Laboratories at Purdue University.

      He is a past President of the International Society for Advancement of Cytometry, is the Editor- in-Chief of Current Protocols in Cytometry, Associate Editor of Histochemica et Cytobiologica, and Associate Editor of Cytometry Part A. He is an active researcher with over 155 peer reviewed publications, 29 book chapters, has edited 9 books and has
      given over 120 international lectures and taught advanced courses in over a dozen countries and made over 350 conference presentations. Dr. Robinson was an early adopter
      of web-based educational materials. He has received numerous awards, was elected to the College of Fellows, American Institute for Medical & Biological Engineering (AIMBE) in 2004, received the Pfizer Award for Innovative Research in 2004 and the Gamma Sigma Delta Award of Merit Research in 2002.

      He has participated in many NIH, NSF and private foundation review boards.


    DATE: May 3, 2017
    TIME: 10:00am PT, 1:00pm ET

    A number of new instruments have appeared in recent times to accommodate different applications and highlight technology advances. This webinar will focus on advances in the Invitrogen™ Attune™ NxT Flow Cytometer and outline where the technology within the Attune NxT fits in current and future applications. 

    I will discuss how the core acoustic focusing technology in the instrument was developed and how it has evolved. Many flow cytometers have unique capabilities and the Attune NxT is no exception. For example, the combination of traditional hydrodynamic focusing in addition to acoustic focusing allows the Attune NxT to achieve some unique tasks such as very high sample flow rates with exceptional CVs.  Everyone knows that flow cytometry offers extreme capability with single cell analysis, but we also know how difficult it is to run very small but valuable samples.  A unique feature of the Attune NxT is that you can dilute that specimen by orders of magnitude and still run the sample in a reasonable time because the sample flow rate can be drastically increased without altering the quality of the data. This feature alone can be responsible for saving critical samples that normally may be lost.  In this regard it is pertinent to understand how and why the Attune NxT fluidics is capable of operating under these conditions and I will explain the fundamentals of the how the high sample flow technology actually works. While discussing technology, I will also explain how the Attune NxT uses a special Coherent designed laser-optics module delivery system to achieve a unique flattop (tophat) focused illumination spot that plays a significant role in the low CVs achieved under high sample flow. I will also discuss my experience with several other aspects of the core technology in the Attune NxT and show how its automated plate reader option can enhance unassisted throughput giving operators back valuable time. 

    For Research Use only.  Not for use in diagnostic procedures.

    Learning Objectives:

    • Compare hydrodynamic and acoustic focusing in flow cytometry
    • List three applications where the Attune NxT provides a unique benefit
    • Describe three automated plate reader options for the Attune NxT


    By following relevant data privacy laws, Thermo Fisher Scientific will take great care in processing my personal data in line with its privacy policy, which can be reviewed at

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