Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimization to prevent nonspecific background effects, artifacts, or inadequate detection by dyes.

This webinar will elucidate these steps as well as the careful precautions you need to take in order to help get specific labeling and publication-quality images to analyze your samples, draw conclusions, and ultimately publish your results.  The webinar walks you through each step, from embedding and running your sample through blocking, antibody labeling, mounting, and imaging the sample. The presenter has first-hand experience developing and using many of these products and draws upon four decades of development of IHC products by Thermo Fisher Scientific.
 

Learning Objectives:

• Learn the aspects of a successful immunohistochemistry protocol.    
• Understand the typical optimizations and troubleshooting issues that may arise during antibody labeling.