MAR 31, 2021 9:00 AM PDT

Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored targeting

Sponsored by: Horizon Discovery
  • Product Manager, Gene Modulation, Horizon Discovery
      Steve Smith joined Horizon Discovery in 2019 and has been part of the company's dedicated focus on providing market-leading tools for examining gene function. At Horizon Discovery, Steve uses his ten years of commercial, scientific experience to help each of our scientific collaborators choose the right tools for their application.
      Steve completed a BS in Biological Sciences and a Ph.D. in Biomedical Sciences from Colorado State University in Ft. Collins, Colorado. His research in Dr. Deborah Roess's lab focused on membrane events during cellular signaling, using biochemical and optical methods. Professionally, his career has focused on tools that study gene functions and microscopy and imaging.

    Date:  March 31, 2021
    Time: 9:00am (PDT),  12:00pm (EDT)
    Knockdown studies are prevalent and powerful ways to illustrate gene function. CRISPRi delivers a new mechanism for gene knockdown, PAM-anchored gene interference without creating double-strand breaks, introducing CRISPR without the cut.
    CRISPRi harnesses the precision of CRISPR to repress gene function at the transcriptional level. We are proud to introduce the first commercially available complete CRISPRi product line, including synthetic single-guide RNA (sgRNA) and a proprietary repressor construct (dCas9-SALL1-SDS3).
    CRISPRi-based gene repression relies on a deactivated Cas9 or dCas9 associated with guide RNA. This complex targets a specific DNA sequence and binds to the DNA downstream of a gene's TSS, blocking gene transcription. The functional result is a depletion of the target protein. Our patent-pending repressor fusion, covalently linked to the dCas9 protein, further inhibits target gene expression by recruiting additional proteins involved in chromatin remodeling and gene silencing.
    CRISPRi is well suited to study any gene and is an ideal platform for simultaneous interrogation of multiple genes. We will share data showing more robust knockdown than demonstrated with existing CRISPRi systems, as well as efficient multiplexing. Importantly, CRISPRi presents another avenue for orthogonal validation studies.
    Join us for this exciting webinar. We will share information about the development of CRISPRi, experimental strategies for successful implementation, and data on the utility and performance of CRISPRmod.
    Topics to be covered
    • What is CRISPRi, and how does it work?
    • Tools needed to perform CRISPR gene inactivation
    • Examples, and data from CRISPRi applications
    • Power of CRISPRi in orthogonal validation
    Webinars will be available for unlimited on-demand viewing after live event.

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