JUN 30, 2020 8:00 AM PDT

Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex Technology

Sponsored by: Leica Biosystems
C.E. Credits: P.A.C.E. CE Florida CE
  • Senior Histotechnologist with GSK R&D
      Chifei is a senior histotechnologist with GSK R&D. He has broad knowledge and technical skills in routine histology, immunohistochemistry, multiplex immunofluorescence, digital pathology and quantitative image analysis. His work scopes from target validation, animal model characterization to clinical biomarker assay development and valiation. He has a master degree in neuorbiology from Fudan Uninersity and a bachelor degree in Biology from Shandong University in China.

    DATE:  June 30, 2020
    TIME:   8:00am PT, 11:00am ET
    Understanding the tumor microenvironment (TME) is essential for the development of effective treatments for cancer patients. Traditional multiplex IHC methods offer limited understanding of the complex TME. This is partially due to technical limitations of chromogenic dyes and fluorescent detection systems. Ultivue DNA barcode labeling technology (InSituPlex) provides an innovative way to detect multiple markers simultaneously in the same tissue section using an automated workflow on the Lecia Biosystems Bond RX platform. This webinar will share our firsthand experience with a two-step 7 marker assay and feature some of the extracted data.
    Learning objectives:
    • Understand the complexity of tumor microenvironment and its importance in drug development
    • Identify the Ultivue DNA barcoding and exchange technology for multiplex immunofluorescence
    • Examine the processes of image fusion, spatial analysis and colocalization of markers on individual cells
    For research use only. Not for use in diagnostic procedures.
    Webinars will be available for unlimited on-demand viewing after live event.
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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