SEP 19, 2019 7:00 AM PDT

Seeing through the murine heart - quantitative 3D assessment of myocardial ischemia/reperfusion injury and response

Sponsored by: Imaris
C.E. Credits: P.A.C.E. CE Florida CE
  • Institute for Experimental Immunology and Imaging, Essen, Germany
      Simon F. Merz is a 3rd year PhD student at the Institute for Experimental Immunology and Imaging under supervision of Prof. Dr. Matthias Gunzer and Prof. Dr. med. Joachim Klode at the University Hospital in Essen, Germany. Before that, he finished both his Bachelor and Master of Science at the University of Heidelberg, the latter with a focus on neuroscience, bringing him in contact with light sheet fluorescence microscopy (LSFM) for the first time. After obtaining the degree, he worked as an application and product specialist for LaVision BioTec GmbH, gaining insights into the Ultramicroscope II and its various applications.

      His research mainly focuses on the application of LSFM and clearing in routine clinical diagnostics. Furthermore, he is involved in a multitude of co-operations based on the use of LSFM and clearing of different tissues and organs from various organisms. Recently, in a close collaboration with the Department of Cardiology and Vascular Medicine, Essen, he published a quantitative imaging approach for the murine heart that allows for homogenous imaging of labelled target structures using antibodies coupled to artificial fluorophores.

      His work has been recognized by a variety of institutions in the form of poster, abstract and presentation awards, travel grants and the selection for an "Image of Distinction" in the Nikon Small World Photomicrography Contest 2018.

    DATE:  September 19, 2019
    TIME:   7:00am PDT, 10:00am EDT, 3:00pm BST
    Cardioprotection by salvage of the infarct-affected myocardium is an unmet yet highly desired therapeutic goal. To develop new dedicated therapies, experimental myocardial ischemia/reperfusion (I/R) injury would require methods to simultaneously characterize extent and localization of the damage and the ensuing inflammatory responses in whole hearts over time. Here we present a three-dimensional (3D), simultaneous quantitative investigation of key I/R injury-components by combining bleaching-augmented solvent-based non-toxic clearing (BALANCE) using ethyl cinnamate (ECi) with light sheet fluorescence microscopy. This allows structural analyses of fluorescence-labeled I/R hearts with exceptional detail. We discover and 3D-quantify distinguishable acute and late vascular I/R damage zones. These contain highly localized and spatially structured neutrophil infiltrates that are modulated upon cardiac healing. Our model demonstrates that these characteristic I/R injury patterns can detect the extent of damage even days after the ischemic index event hence allowing the investigation of long-term recovery and remodeling processes.
    Learning Objectives:
    • Insights into anatomical structure of the murine heart in 3D
    • Immune response following ischemia/reperfusion injury on an organ level
    • Quantitative light sheet fluorescence microscopy (LSFM) in highly autofluorescent organs
    • Examples for semi-manual tracing, spot-function and filament model to quantify 3D LSFM data
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