Recent revolutionary developments in the field of cryo electron microscopy (EM) revealed cellular mechanisms in subnanometer resolution. Unfortunately, it is very challenging to identify and localize structures of interest in the cryo TEM alone. To facilitate this, cryo workflows have been established integrating cryo light microscopes for sample assessment and target identification. The THUNDER Imager EM Cryo CLEM is a cryo light microscope used to identify and mark fluorescent structures in vitrified cellular samples for easy retrieval in the subsequent cryo EM preparation steps. The THUNDER technology employs the innovative Leica method of Computational Clearing to eliminate out-of-focus blur, that can occur with widefield observation. The result is crisp, haze-free images for more precise identification of cellular target structures.

 

This seminar presents the system and it´s integration into state-of-the-art cryo EM workflows.

 

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