APR 02, 2020 8:00 AM PDT

Bionano Genome Imaging unravels complex structural rearrangements induced by replication stress in liver cancer

Sponsored by: Bionano Genomics
C.E. Credits: P.A.C.E. CE Florida CE
  • Functional Genomics of Solid Tumors , Centre de Recherche des Cordeliers, Paris
      After a general training in life sciences at the Ecole Normale Supérieure of Lyon, Eric Letouze specialized in the bioinformatic analysis of high-throughput genomic data, applied to cancer research. During his PhD at Institut Curie and as a post-doc at the Ligue Nationale Contre le Cancer, he was involved in the identification of novel therapeutic targets and molecular markers of prognosis.He also designed innovative computational tools for the analysis of high-throughput genomic data. He joined the INSERM team "Genomics of liver and mesothelial tumors", led by Jessica Zucman-Rossi, in May 2014 to develop a new research area on the genetic and epigenetic signatures of oncogenic processes, with an emphasis on liver tumors.

      His research project aims at understanding how the interaction of the genetic background and environmental exposures lead a healthy cell to become malignant, and how the genetic diversity of liver tumors is established. To do so, his team develops innovative computational approaches to extract biological insights from large genomic datasets, including whole-exome and whole-genome sequencing,RNA-sequencing and DNA methylation data. His current projects include identifying rare variants predisposing to liver cancer in whole-exome data, analyzing mutational signatures and the clonal architecture of tumors using whole-genome data, or exploring the transcriptome of liver tumors and its interaction with epigenetic alterations.

    DATE:  April 2, 2020
    TIME:  8:00am PT, 11:00am ET, 5:00pm CET
    Cyclins A2 and E1 regulate the cell cycle by promoting S phase entry and progression. We recently identified a hepatocellular carcinoma (HCC) subgroup exhibiting cyclin activation through various mechanisms, including HBV and AAV2 viral insertions, gene fusions and enhancer hijacking. Those poor-prognosis HCCs display a unique signature of structural rearrangements, triggered by replicative stress. This signature is strongly enriched in early-replicated active chromatin regions and is characterized by hundreds of tandem duplications and more complex events called Templated Insertion Cycle (T.I.C.).
    Structural variation calling from short-read Whole Genome Sequencing provides abnormal junctions by comparing chimeric reads with a reference genome. However, those independent breakpoints are too distant, thus this method is not enough to reconstruct highly complex rearrangements, which may involve up to dozens of regions of the genome linked together. Here we used Bionano data to characterize with certainty large DNA molecules resulting from complex T.I.C.. This analysis allowed us to know which regions of the genome are the acceptor of such complex structural rearrangements. This information is critical in the understanding of how those rearrangements affect genes involved in tumorigenesis by placing oncogenes in different genomic contexts.
    In this webinar, you can learn more about:
    • Optical mapping with Bionano Genome Imaging workflow
    • How optical mapping can uncover novel structural variants as well as complex rearrangements genome-wide and in an unbiased fashion
    • Data on how optical mapping is a complementary technique to short and long read sequencing
    • Data how complex rearrangements were uncovered in hepatocellular carcinoma
    Webinars will be available for unlimited on-demand viewing after live event.
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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