DATE: August 22, 2017
TIME: 9:00am PT, 12:00pm ET
The use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gene editing using the homology-directed repair (HDR) mechanism. This approach enables knock-in of fluorescent reporters, SNP repair, exact deletions, and many other genomic alterations. However, designing target-specific guide RNAs and DNA donor templates for achieving robust cleavage and repair has many challenges. In addition, the efficiency of HDR-mediated repair is often low. In this workshop, we will demonstrate the use of free, intuitive, online tools to design and order the necessary reagents to overcome these obstacles with HDR-mediated gene editing applications.