Education

Each of these modular, animated, narrated eLearning courses were developed to provide a succinct, contextual summary of common laboratory techniques and methods used by researchers with an interest in protein and cell analysis workflows. At the end of each course, take an opportunity to apply the theory that was provided by completing the associated practical application exercises. We then invite you to download the eLearning certificate of completion for each course that you master.

If you have already registered for the Protein and Cell Analysis Education platform, you simply need to click on the “Start the course” link for the course you are interested in and log in with your credentials.

If you have not registered for the Protein and Cell Analysis Education platform or are not a LabRoots member already, you will need to first sign up by clicking the SIGN UP button. Once you have completed that form, you can click on the “Start the course” link and sign in.

 

eLearning course

Topics covered

T Cell Stimulation and Proliferation

 

 

 

Part 1: T cell biology in translational biomedical science; basic T cell biology in the context of the wider immune system; T cell structure and function; T cell receptor signaling; T cell activation and proliferation (antigen presenting cell interactions and T effector functions; T cell subsets (including subset-specific signature proteins)

Part 2:  T cell isolation and in vitro activation; assessment of T cell activation, proliferation, and differentiation by these methods:

  • Flow cytometric analysis
  • Enzyme-linked immunosorbent assays
  • Multiplex Luminex™ assays

 

Practical Application —Flow Cytometry

 

Start the course   

 

eLearning course

Topics covered

Protein Sample Preparation: Techniques and Applications

 

 

 

 

 

 

 

 

Part 1: Total protein extraction; cell disruption methods: (reagent-based and physical); subcellular fractionation; sample preparation for downstream assays such as:

  • Western blotting
  •  ELISA
  • Immunoprecipitation
  • Mass spectrometry

 

Practical Application—Part 1: Protein Isolation

Part 2:  Hydrophobic interaction chromatography (HIC); size exclusion chromatography (SEC); ion exchange chromatography (IEX); affinity chromatography (AC); protein cleanup methods:

  • Desalting
  • Buffer exchange
  • Dialysis

 

Practical Application—Part 2: Protein Purification

 

Start the course   

 

 

 

 

For research use only. Not for use in diagnostic procedures.

APR 10 2017 - MAY 30 2018
Protein and Cell Analysis Education Series
18 61 13807

The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mass spectrometry, flow cytometry, fluorescence imaging, high content imaging, antibodies and antibody labeling, multiplex immunoassays, and more.

 
The webinars and other educational content are provided by the scientists that developed the Molecular Probes™ Handbook and School of Fluorescence and the Pierce™ Protein Methods Handbook.  This includes:

  •     Access to animated and narrated eLearning courses and practical application modules
  •     A wide selection of technical handbooks & white papers
  •     How-to & educational videos about techniques and applications
  •     On demand webinars & tutorials covering the basics of protein and cell analysis
  •     Easy access to content in our Thermofisher.com Learning Centers

 

When you register, you will also get notification of upcoming webinars and other activities. All content is available 24/7 and is viewable from the convenience of your desk, tablet or mobile device. Sign up today!

 

 

For research use only. Not for use in diagnostic procedures.


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Thermo Fisher Scientific Inc. is the world leader in serving science, with revenues of $18 billion and more than 55,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive support.

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