NOV 20, 2019 8:00 AM PST

Approaches to nucleic acid isolation: from sample to NGS analysis

Sponsored by: Cytiva
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • NGS Core Facility Manager, Private Molecular Diagnostic Lab, Cologne, Germany.
    Biography
      Dr Stefanie Stepanow is an NGS core facility manager in a private molecular diagnostic lab in Cologne, Germany. She is responsible for the establishment and validation of new NGS assays as well as monitoring and optimization of current NGS workflows. For almost 9 years, Stefanie has worked routinely with different NGS techniques (Illumina™, Ion Torrent™) and a wide range of sample materials while gaining experience in optimizing NGS workflows to achieve meaningful results from difficult materials. Previously, she worked as a scientist in an NGS core facility with focus on clinical and biological research in Duesseldorf, where she established and standardized the NGS techniques and attained lab accreditation.

    Abstract
    DATE:   November 20, 2019
    TIME:   8:00am PST, 11:00 EST, 4:00pm GMT, 5:00pm CET
     
    Even as next-generation sequencing (NGS) has become widely used, and the costs have been reduced, it remains critical to achieve reliable and robust data at the end of the sequencing run. Sequencing labs have very little control over the quantity and quality of the sample received from the pathology lab or clinic, yet sample quality can have a major impact on the downstream processes.
     
    While the manual, column-based approach to nucleic acid isolation is still widely used, scientists under pressure from high-throughput demands are turning their attention to superparamagnetic beads. These are automation friendly and well-suited for a range of applications, including sample preparation for next generation sequencing (NGS). Each technique has pros and cons, and selection will depend on sample type, throughput requirements, targets, and other factors.
     
    Join Dr. rer. nat. Stefanie Stepanow on November 20, 2019 for a free webinar reviewing the column and bead-based approaches to nucleic acid isolation for DNA in molecular diagnostic applications and optimization of the process from sample assessment to NGS analysis.
     
    In this webinar you will:
    • Demonstrate how to check sample quality against set criteria and assess the potential impact on data outcomes in relation to genomic and prenatal testing applications
    • Review modifications to workflows, protocol steps and NGS analysis to manage the quality of complex samples such as FFPE and liquid biopsy
    • Compare the benefits and drawbacks of different approaches to nucleic acid isolation with particular focus on column-based and magnetic bead-based approaches
    • Review sequencing data comparing column-based and bead-based approaches
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    NOV 10, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 10, 2020 7:00 AM PST
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    JUN 23, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    Loading Comments...
    Show Resources
    Attendees
    • See more