Significant improvements in a chromatography workflow can be achieved with small changes and proactive choices.
Before starting protein purification, it is important to define your requirements and objectives. These may include outlining the required purity, activity and the quantity of the target you want to achieve, ensuring process steps and handling are kept to a minimum and all critical impurities are removed.
This educational session is aimed to give guidance to protein purification protocol development for laboratory scale, and is designed for researchers working with either tagged or native proteins.
A key component of this Webinar is the introduction of a proven protein purification strategy, called CiPP (Capture, Intermediate purification, Polishing). It explains how to best combine the main separation techniques in order to obtain a powerful purification protocol delivering the pure and active protein you need for your work.