FEB 20, 2017 08:00 AM PST
How to combine protein purification techniques to optimize your results
Presented at the Protein Research Live Virtual Event
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Speakers:
  • Research Director, GE Healthcare Life Sciences, Sweden
    Biography
      Åke was born in 1953 in Stockholm, Sweden. He graduated from Uppsala University with a M.Sc. in Chemistry / Biology in 1978. He obtained his Ph.D. in veterinary medical chemistry at the Swedish University of Agricultural Sciences, in Uppsala, in 1982. He held a position as Research Associate at the Swedish University of Agricultural Sciences between 1982 and 1986, studying the molecular mechanisms of blood coagulation. He has published several papers on the interaction of the plasma protein inhibitor, antithrombin with heparin and coagulation enzymes. In 1987, Åke joined Pharmacia LKB Biotechnology, which later became GE, in Uppsala. Åke has held a number of different R&D positions in GE Healthcare Life Sciences, most recently as a Staff Scientist and now as Research Director. He has been involved in the development of a number of chromatography resins for protein separations and of the ÄKTATM chromatography systems product line.

    Abstract:

    Significant improvements in a chromatography workflow can be achieved with small changes and proactive choices.


    Before starting protein purification, it is important to define your requirements and objectives. These may include outlining the required purity, activity and the quantity of the target you want to achieve, ensuring process steps and handling are kept to a minimum and all critical impurities are removed.


    This educational session is aimed to give guidance to protein purification protocol development for laboratory scale, and is designed for researchers working with either tagged or native proteins.


    A key component of this Webinar is the introduction of a proven protein purification strategy, called CiPP (Capture, Intermediate purification, Polishing). It explains how to best combine the main separation techniques in order to obtain a powerful purification protocol delivering the pure and active protein you need for your work.

     


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