OCT 27, 2016 08:00 AM PDT

CRISPR-Cas9: From reagent selection to experimental optimization

Speakers
  • Product Manager; Dharmacon, part of GE Healthcare
    Biography
      James Goldmeyer, PhD, Product Manager at Dharmacon, part of GE Healthcare. James joined Dharmacon in 2009 and has lead the development and commercial launch of numerous genome-wide shRNA and gene-overexpression product lines. He also shares responsibility for, and is heavily involved in the development, of the Dharmacon Edit-R Dharmacon Gene Engineering portfolio; with emphasis on the vector-based products for lentiviral sgRNA and Cas9 nuclease expression. James works closely with an expert group of R&D scientists on innovative tools and services for gene modulation and engineering. James earned a doctorate degree in physiology and neurobiology at the University of Connecticut.

    Abstract:
    DATE:  October 27, 2016
    TIME:   8:00am PT, 11:00am ET

    The use of CRISPR-Cas9 for gene editing has opened up many new avenues for scientific exploration around gene function. The rapid expansion of the field has led to a wide range of technology formats for use in both gene knockout and precision knockin genome engineering experiments. The seemingly ever-increasing toolset has also led to a widening knowledge gap for newcomers to the field to overcome in determining the proper reagents for performing experiments.

    During this webinar we will discuss the basics of CRISPR-Cas9 gene editing and the key criteria and decision points in selecting reagents based on your desired application. We will review all types of guide RNA and Cas9 nuclease formats and discuss the advantages and disadvantages of each.

    Furthermore, we will present techniques for optimizing experimental conditions to maximize success, including functional knockout and HDR for knockin. To provide context for the selection of different formats, we will present data showing direct comparison of Cas9 nuclease and guide RNA formats under different experimental protocols.

    In this webinar you will learn:
    • Criteria for selection of CRISPR-Cas9 components
    • Importance of guide RNA rational design for functional and specific knockout
    • Optimizing delivery of CRISPR-Cas9 components

    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    JUN 29, 2018 09:00 AM PDT
    C.E. CREDITS
    JUN 29, 2018 09:00 AM PDT
    DATE: June 29, 2018TIME: 09:00AM PDT, 12:00PM EDT There is significant epidemiological evidence to suggest that the consumption of a high-broccoli diet is associated with a r...
    AUG 22, 2017 09:00 AM PDT
    C.E. CREDITS
    AUG 22, 2017 09:00 AM PDT
    DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
    Loading Comments...