MAY 19, 2015 8:00 AM PDT

How to combine protein purification techniques to optimize your results

Sponsored by: GE Healthcare, GE Healthcare
Speaker
  • Research Director, GE Healthcare Life Sciences, Sweden
    Biography
      Åke was born in 1953 in Stockholm, Sweden. He graduated from Uppsala University with a M.Sc. in Chemistry / Biology in 1978. He obtained his Ph.D. in veterinary medical chemistry at the Swedish University of Agricultural Sciences, in Uppsala, in 1982. He held a position as Research Associate at the Swedish University of Agricultural Sciences between 1982 and 1986, studying the molecular mechanisms of blood coagulation. He has published several papers on the interaction of the plasma protein inhibitor, antithrombin with heparin and coagulation enzymes. In 1987, Åke joined Pharmacia LKB Biotechnology, which later became GE, in Uppsala. Åke has held a number of different R&D positions in GE Healthcare Life Sciences, most recently as a Staff Scientist and now as Research Director. He has been involved in the development of a number of chromatography resins for protein separations and of the ÄKTATM chromatography systems product line.

    Abstract

    Sign up here to watch this Webinar On Demand


    Click here to check out the full Q&A session from our protein purification webinar!


    Significant improvements in a chromatography workflow can be achieved with small changes and proactive choices.


    Before starting protein purification, it is important to define your requirements and objectives. These may include outlining the required purity, activity and the quantity of the target you want to achieve, ensuring process steps and handling are kept to a minimum and all critical impurities are removed.


    This educational session is aimed to give guidance to protein purification protocol development for laboratory scale, and is designed for researchers working with either tagged or native proteins.


    A key component of this Webinar is the introduction of a proven protein purification strategy, called CiPP (Capture, Intermediate purification, Polishing). It explains how to best combine the main separation techniques in order to obtain a powerful purification protocol delivering the pure and active protein you need for your work.


    Show Resources
    You May Also Like
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    JUN 09, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    MAR 03, 2020 9:00 AM JST
    C.E. CREDITS
    MAR 03, 2020 9:00 AM JST
    DATE: March 3, 2020 TIME: 9:00am JST A major limitation in the ex vivo expansion of harvested human hematopoietic stem-progenitor cells (HSPCs) is the rapid differentiation of HSPCs at the e...
    FEB 25, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 25, 2020 9:00 AM PST
    Learn about how to generate a small scale CAR-T workflow using ThermoFisher products See detailed characterization tools that can be utilized and applied in a CAR-T workflow...
    Loading Comments...
    Show Resources
    Attendees
    • See more