MAY 19, 2015 08:00 AM PDT

How to combine protein purification techniques to optimize your results

SPONSORED BY: GE Healthcare, GE Healthcare
Speakers
  • Research Director, GE Healthcare Life Sciences, Sweden
    Biography
      Åke was born in 1953 in Stockholm, Sweden. He graduated from Uppsala University with a M.Sc. in Chemistry / Biology in 1978. He obtained his Ph.D. in veterinary medical chemistry at the Swedish University of Agricultural Sciences, in Uppsala, in 1982. He held a position as Research Associate at the Swedish University of Agricultural Sciences between 1982 and 1986, studying the molecular mechanisms of blood coagulation. He has published several papers on the interaction of the plasma protein inhibitor, antithrombin with heparin and coagulation enzymes. In 1987, Åke joined Pharmacia LKB Biotechnology, which later became GE, in Uppsala. Åke has held a number of different R&D positions in GE Healthcare Life Sciences, most recently as a Staff Scientist and now as Research Director. He has been involved in the development of a number of chromatography resins for protein separations and of the ÄKTATM chromatography systems product line.

    Abstract:

    Sign up here to watch this Webinar On Demand


    Click here to check out the full Q&A session from our protein purification webinar!


    Significant improvements in a chromatography workflow can be achieved with small changes and proactive choices.


    Before starting protein purification, it is important to define your requirements and objectives. These may include outlining the required purity, activity and the quantity of the target you want to achieve, ensuring process steps and handling are kept to a minimum and all critical impurities are removed.


    This educational session is aimed to give guidance to protein purification protocol development for laboratory scale, and is designed for researchers working with either tagged or native proteins.


    A key component of this Webinar is the introduction of a proven protein purification strategy, called CiPP (Capture, Intermediate purification, Polishing). It explains how to best combine the main separation techniques in order to obtain a powerful purification protocol delivering the pure and active protein you need for your work.


    Show Resources
    You May Also Like
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    SEP 12, 2017 08:00 AM PDT
    SEP 12, 2017 08:00 AM PDT
    DATE: September 12, 2017TIME: 8:00am PT, 11:00am ETEvery year, millions of dollars are wasted on poorly characterized and performing antibodies.  Key researchers in the antibody co...
    MAY 24, 2018 09:00 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:00 AM PDT
    Recent advances in immuno-oncology research has proven the critical role of immune cells in cancer therapy. Deep understanding of the interaction and phenotype of immune and tumor cells withi...
    Loading Comments...