Mechanisms of Myeloid Immunosuppression: Functional Characterization of the Tumor Microenvironment Using Single and Multiplex IHC

Sponsored by: Cell Signaling Technology

Speaker

Event Date & Time

DATE: October 3, 2018

TIME: 9:00am PDT, 12:00pm EDT

Abstract

Infiltrating myeloid cells constitute a significant component of the suppressive tumor microenvironment (TME) and possibly contribute to therapeutic failures. An understanding of the immune regulatory context of the TME is required to harness the power of the antitumor immune response. Here, we present solutions to better understand the TME in a context-dependent manner. Using highly validated antibodies, immunohistochemical techniques are employed in a seven-color TSA-based multiplex panel along with single and dual-plex chromogenic stains to visualize biomarker expression in an array of tumor types. Newly validated Arginase1 and IDO1 monoclonal antibodies add a functional readout of the immunosuppressive TME to phenotypic marker analysis.

Learning Objectives: