MAR 23, 2017 8:00 AM PDT

WEBINAR: Using arrayed synthetic CRISPR libraries for high-throughput functional genomics studies

Sponsored by: GE Healthcare
Speaker
  • Director of R&D, Dharmacon, part of GE Healthcare
    Biography
      Anja Smith is the Director of R&D at Dharmacon, part of GE Healthcare. She joined Dharmacon in 2005 and currently manages a team of R&D and Technical Support scientists with expertise in bioinformatics, chemistry, and molecular and cellular biology. Anja oversees projects focused on innovation and new product development in multiple research areas including RNA interference and CRISPR-Cas9 genome engineering. She received her Bachelor of Science degree in Biology at Yale University and her Ph.D. degree in Genetics at the University of Washington. Anja completed a postdoctoral fellowship at Memorial Sloan-Kettering Cancer Center.

    Abstract

    DATE: March 23, 2017
    TIME: 8:00am PT, 11:00am ET

    The use of CRISPR libraries containing algorithm-designed synthetic crRNAs improves the likelihood of functional gene knockout and can be very easy to deliver with standard transfection methods. By performing the crRNA screen in an arrayed, one-gene-per-well format, the types of assays that can be used are nearly limitless! For example, high-content assays can explore complex phenotypes including intracellular localization and cellular morphology. In this webinar, we will show you how to be successful with an arrayed crRNA screen and we’ll show you the types of assays that have been used so far. We will also share results from our high-content arrayed crRNA screen and review screening results from recent publications. 

    Learning Objectives:

    • Benefits of performing arrayed CRISPR RNA (crRNA) screening
    • How to setup a successful arrayed crRNA screen
    • Examples of functional analysis assays for arrayed screening
    • Results from a multiparametric high-content screen

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