MAR 23, 2017 08:00 AM PDT

WEBINAR: Using arrayed synthetic CRISPR libraries for high-throughput functional genomics studies

SPONSORED BY: GE Healthcare
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Director of R&D, Dharmacon, part of GE Healthcare
    Biography
      Anja Smith is the Director of R&D at Dharmacon, part of GE Healthcare. She joined Dharmacon in 2005 and currently manages a team of R&D and Technical Support scientists with expertise in bioinformatics, chemistry, and molecular and cellular biology. Anja oversees projects focused on innovation and new product development in multiple research areas including RNA interference and CRISPR-Cas9 genome engineering. She received her Bachelor of Science degree in Biology at Yale University and her Ph.D. degree in Genetics at the University of Washington. Anja completed a postdoctoral fellowship at Memorial Sloan-Kettering Cancer Center.

    Abstract:

    DATE: March 23, 2017
    TIME: 8:00am PT, 11:00am ET

    The use of CRISPR libraries containing algorithm-designed synthetic crRNAs improves the likelihood of functional gene knockout and can be very easy to deliver with standard transfection methods. By performing the crRNA screen in an arrayed, one-gene-per-well format, the types of assays that can be used are nearly limitless! For example, high-content assays can explore complex phenotypes including intracellular localization and cellular morphology. In this webinar, we will show you how to be successful with an arrayed crRNA screen and we’ll show you the types of assays that have been used so far. We will also share results from our high-content arrayed crRNA screen and review screening results from recent publications. 

    Learning Objectives:

    • Benefits of performing arrayed CRISPR RNA (crRNA) screening
    • How to setup a successful arrayed crRNA screen
    • Examples of functional analysis assays for arrayed screening
    • Results from a multiparametric high-content screen

    Show Resources
    You May Also Like
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    JUN 29, 2018 09:00 AM PDT
    C.E. CREDITS
    JUN 29, 2018 09:00 AM PDT
    DATE: June 29, 2018TIME: 09:00AM PDT, 12:00PM EDT There is significant epidemiological evidence to suggest that the consumption of a high-broccoli diet is associated with a r...
    APR 26, 2018 07:30 AM PDT
    C.E. CREDITS
    APR 26, 2018 07:30 AM PDT
    DATE: April 26, 2018TIME: 07:30am PDT, 04:30pm CESTThe NGS library preparation process is labor-intensive, requiring experience and attention to detail over hours. Eppendorf consumables...
    Loading Comments...