The CRISPR/Cas system is not highly biocompatible with isothermal nucleic acid amplification methods, requiring sequential reactions, which may induce aerosol contamination and false-positive signals. To address this challenge, we introduced a physical barrier device to achieve spontaneous dynamic fusion of two separated systems, establishing a one-step CRISPR/Cas pathogen detection platform with spatiotemporal dual-dimensional separation, thereby resolving issues of aerosol contamination and system incompatibility.

Learning Objectives:

1. Explain how the incompatibility between CRISPR/Cas systems and isothermal nucleic acid amplification methods can lead to false-positive signals in pathogen detection.

2. Describe the design and function of the physical barrier device that enables spatiotemporal dual-dimensional separation in a one-step CRISPR/Cas detection platform.

3. Analyze the impact of the one-step CRISPR/Cas platform on reducing aerosol contamination and improving system compatibility in molecular diagnosis and infectious disease prediction.