MAY 18, 2017 08:00 AM PDT

WEBINAR: Create Your Own Cellular Compound-Target Engagement Assay

C.E. CREDITS: P.A.C.E. CE | Florida CE
  • Sr. Product Manager, DiscoverX Corporation
      Dana Haley-Vicente, Ph.D., is currently a Sr. Product Manager at DiscoverX (Fremont, CA) managing cell-based assays, kits, and consumable products for drug discovery target discovery, screening, lead optimization, and SAR campaigns. She has been working in the life science drug discovery industry for over 16 years and received her Ph.D. in Pharmacology from University of California, Los Angeles.


    DATE: May 18, 2017
    TIME: 8:00AM PDT, 11:00AM ET

    It is well established that ligand binding can protect proteins from thermal denaturation in a cellular milieu; however, the broad application of this cellular target engagement concept to drug discovery has been hindered by a lack of simple platforms with sensitive quantitative readouts. Cell-based thermal stabilization assays are valuable methods for particular applications, but they can require target-specific antibodies for immuno-assay readouts, can be low-throughput for Western blot formats, and can be biased against multi-domain proteins. 
    Here we will describe InCELL Pulse™, which uses DiscoverX's Enzyme Fragment Complementation (EFC) technology to overcome these limitations of current thermal stabilization assays. This unique platform allows you to rapidly measure quantitative cellular target engagement potency values for inhibitors of diverse protein classes, including kinases, methyltransferases, and hydrolases. The tools and workflow for designing your own InCELL Pulse assays for your favorite target will be described and validation data will be highlighted.

    Learning Objectives:

    • Overview of compound-target engagement
    • Tools and workflow to create your own cell-based target engagement assay
    • Applications of InCELL Pulse assays

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