JUN 14, 2017 6:00 AM PDT

WEBINAR: Accelerated Data Workflows for Biomarker Discovery in Metabolomics: Clinical Research Applications in Cystic Fibrosis Research

Sponsored by: Agilent
  • Professor and Cystic Fibrosis Canada Researcher; Dep. Chemistry & Chemical Biology, McMaster University
      Philip Britz-McKibbin is a Professor at the Department of Chemistry and Chemical Biology and Cystic Fibrosis Canada Researcher based at McMaster University. Prior to starting his current position, he completed his doctorate research with David D.Y. Chen at the Univ. of British Columbia (2000) followed by a visiting lecturer position at the Univ. of North Carolina at Greensboro in USA (2001) and a post-doctoral fellowship with Shigeru Terabe at Himeji Institute of Technology in Japan (2003). His research interests in bio-analytical chemistry and metabolomics include the design of new analytical strategies to quantify and identify metabolites in biological samples using innovative separation, mass spectrometric and bioinformatic tools. Philip's laboratory aims to discover new biochemical markers that support early detection and treatment of human diseases relevant to population/occupational health and pediatric/perinatal medicine with emphasis on inherited metabolic disorders and the developmental origins of health and disease. His research has been funded by NSERC, CFI, CIHR, Cystic Fibrosis Foundation of Canada, the Ontario Genomics Institute, and the Ontario Ministry of Labour. He has presented over 100 invited talks and published more than 70 peer-reviewed articles, including 6 invited reviews, 5 book chapters, 2 technical reports and 4 filed patents with 2 licenses. Dr. Britz-McKibbin is the recipient of several prestigious awards, including the American Chemical Society - Young Investigator Award in Separation Science (2010), Japan Society for Promotion of Science (2009), Petro-Canada Young Investigator Award (2007) and Premier's Research Excellence Award (2004). Philip is a recent Evaluation Group Member in Chemistry for NSERC Discovery Grant program, an editorial board member in Chemical Biology of Scientific Reports, a Founding Advisory Board Member of the US Chapter of the Metabolomics Society and an affiliate member of the Metabolomics Innovation Centre.


    DATE: June 14, 2017
    TIME: 6:00am PT, 9:00am ET, 3:00pm CET

    Comprehensive metabolite profiling (i.e., metabolomics) offers a holistic approach for understanding the phenotype of an organism on a molecular level that is closely associated with clinical research outcomes. However, low sample throughput and complicated data processing remain major bottlenecks to biomarker discovery in clinical-based metabolomics research. An introduction to multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS) will first be presented as a multiplexed separation platform that takes advantage of customized serial injections to enhance sample throughput and data fidelity with quality assurance. Various sample injection formats can be designed to encode MS information temporally when using signal pattern recognition for unambiguous biomarker identification when analyzing volume-restricted biological samples, including dried blood spot extracts and sweat specimens. Structural elucidation of unknown metabolites can be deduced with greater confidence when using high resolution tandem MS experiments in conjunction with CE-based mobility theory.  Recent progress towards characterization of the sweat metabolome of screen-positive cystic fibrosis infants, as well as identification of new markers of CF in asymptomatic neonates will be discussed in support of universal newborn screening programs. MSI-CE-MS offers a cost-effective approach for greatly expanding the productivity of MS-based chemical analyses while offering an accelerating data workflow for biomarker discovery in metabolomics.

    For Research Use Only. Not for use in diagnostic procedures.

    Learning Objectives:

    • What is metabolomics?: Non-targeted metabolite profiling for discovery-based research in clinical medicine
    • What is in human sweat?: Using CE-MS-based metabolomics for comprehensive characterization of volume-restricted biospecimens
    • Beyond sweat chloride: How can sweat biomarkers improve diagnostic testing and/or treatment monitoring of cystic fibrosis patients?

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