MAY 09, 2019 10:30 AM PDT

Background and Troubleshooting for RT-PCR

Sponsored by: MilliporeSigma
Speaker

Abstract

Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle.  For quantitative reverse transcription PCR (RT-qPCR), the starting material is RNA, which is then transcribed to cDNA by a reverse transcriptase prior to the qPCR assay. Gene expression is a common application for qPCR, and amplification through RT-qPCR is necessary to detect and quantify gene expression from small amounts of RNA. This webinar will present the basics of RT-qPCR and tips and tricks for performing RT-qPCR experiments.

Learning Objectives: 

1. Understand the basics of RT-PCR, including how it is used for quantitating gene expression and its advantages over other methods
2. Explore successful optimization strategies and troubleshooting tips to achieve better results


MAY 09, 2019 10:30 AM PDT

Background and Troubleshooting for RT-PCR

Sponsored by: MilliporeSigma