OCT 11, 2017 10:30 AM PDT

Characterization and application of the triple immunodeficient R2G2 (Rag2/Il2rg double knockout) mouse model

Sponsored by: Envigo
  • Chief Scientific Officer - Biologics, Envigo
      Lee was recruited by Huntingdon Life Sciences at the end of 2004 to take a leading role in working with customers developing biopharmaceutical products.

      Lee brought to the role hands-on experience of biopharmaceutical drug development gained in companies such as; Cantab Pharmaceuticals, Xenova and CellFactors. During his time in the biotechnology industry, he was involved in developing therapies including: vaccines, recombinant proteins; monoclonal antibodies; immunomodulatory molecules; viral and non-viral gene delivery systems; and cell-based therapies.

      He has a well-rounded knowledge of the regulatory environment for biologics and particular expertise in the analysis of protein and viral products.

      At Envigo, Lee is Chief Scientific Officer - Biologics. He is also a serving committee member on the Monoclonal Antibody Expert Working Group of the National Centre for the 3Rs, The BIA Manufacturing Advisory Committee, the RSC / RPS Joint Pharmaceutical Analysis Group the ABPI Nonclinical and Biological Discovery Expert Network (NaBDEN) committee.


    The “R2G2” (B6;129-Rag2tm1FwaIL2rgtm1Rsky/DwlHsd) knockout mouse is the latest advancement to provide an alternative option in the highly immunodeficient mouse model category for oncology, immunology and other biomedical research communities. This model was generated by backcrossing an IL2rg (common gamma) knockout model to a RAG2 (recombinase activating gene) knockout model. The resulting mouse lacks various cytokines including IL-2, IL-4, IL-7, IL-9 and IL-15. In addition, this model lacks B cells, T cells, NK cells and has a deficiency in lymphocyte development. The R2G2 mouse model is not only ultra immunodeficient, but provides a model that is less leaky and more tolerant to gamma radiation than traditional SCID models. Herein we describe the development and characterization of the R2G2 mouse model, which includes breeding history, growth curve, complete blood count and serum chemistry, flow cytometry, tumor growth and radiosensitivity.

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