FEB 07, 2018 10:30 AM PST

Evaluation of Analgesia Efficacy in Postoperative Mice Using Novel Cage-Side Measures

Speaker

Abstract

The ability to rapidly and accurately identify pain in mice is critical for providing them optimal care and welfare. To meet this need, we developed and validated the Grooming Transfer Test (GTT) and Nest Consolidation Test (NCT). We assessed these novel tests along with electronic von Frey and ambulatory parameters at baseline, after isoflurane anesthesia +/- analgesia, and after laparotomy in adult CD1 and C57BL/6 mice of both sexes, housed singly with or without an existing nest or in pairs. While ambulatory parameters had no and von Frey responses minimal significant changes after surgery, GTT and NCT were significantly altered for 48 hours after surgery in both sexes, strains, and across the various housing conditions. Buprenorphine and carprofen each reduced post-operative pain, however only the combination of the two completely prevented delays in nesting behavior. Therefore, these two novel cage-side methods can be used to quickly and objectively identify mice from a variety of signalments and housing conditions with alleviated and unalleviated postoperative pain. 


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FEB 07, 2018 10:30 AM PST

Evaluation of Analgesia Efficacy in Postoperative Mice Using Novel Cage-Side Measures


Specialty

Animal Models

Animal Research

Life Science

Neuroscience

Research

Veterinary Sciences

Animal Sciences

University

Cell Biology

Research And Development

Disease

Animal Behavior

Animals

Cell

Cancer

Geography

Europe84%

North America7%

Asia5%

Registration Source

Website Visitors100%

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Medical Doctor/Specialist17%

Student17%

Medical Laboratory Technician17%

Scientist11%

Post Doc11%

Executive6%

Facility/Department Manager6%

Educator/Faculty6%

Research Scientist3%

Consultant3%

Veterinary Technician3%

Organization

Academic Institution57%

Hospital16%

Research Institute7%

Pharmaceutical Company5%

Consultant2%

Manufacturer - Other2%

Medical School2%

Contract Research Organization (Cro)2%

Medical Center2%


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