DEC 04, 2020 9:00 AM PST

IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail - Visualizing immune response during infections

Speaker
  • Ph.D.
    Biography

      Dr. Shankar Pattabhiraman is a Technical Product manager for the Flow Cytometry business unit with Beckman Coulter Life Sciences, where he specializes in the development of multicolor flow cytometry applications for research and clinical use. He holds a Ph.D. in human medicine from Charite Medical University Berlin, Germany, and previously served as a Research Scientist with INSERM, France. His current interests are in the immunology of infections, and cell therapy solutions.


    Abstract

    Date: December 4, 2020

    Time: 9:00am PST, 12:00pm EST

    Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives an excellent overview of the innate immune response. The flow cytometry assay can effectively differentiate the immune response to bacteria or viruses.
    Bacterial stimuli: CD64 overexpression on neutrophils (nCD64).
    Viral stimuli: CD169 overexpression on monocytes (mCD169).
    Recent stimulus: increased HLA-DR, Overstimulation/Immune exhaustion: decreased HLA-DR.
    Further, the assay, using whole blood, is rapid and easy to perform, making this an invaluable tool in clinical research on antibiotic resistance, and studying interferon signaling during infections.


     
    Learning Objectives:

    • Describe Myeloid cell activation and how it is specific for viral and bacterial infections due to differential interferon signaling.
    • Explain how Markers CD64 and CD169 can be effectively used to differentiate between the types of infections.

     

     

    * For Research Use Only. Not for use in diagnostic procedures.

     

     

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