A recently proposed model for the incorporation of xenobiotics of clinical research and forensic interest into the human skeleton suggests nerve agent metabolites may incorporate into bone at relatively elevated levels based on their unique chemical properties. To assess this possibility, methods for the extraction, isolation, and semi-quantitative detection of nerve agent metabolites (corresponding to VX, Russian VX, sarin, cyclosarin, and soman) from bone were developed using liquid chromatography mass spectrometry with both quadrupole time-of-flight (QToF) and triple quadrupole (QqQ) instruments.

Learning Objectives:

1. Understand LC-HRMS method development

2. Identify the clinical research of bone

For Research Use Only. Not for use in diagnostic procedures.