JUL 21, 2020 8:00 AM PDT

Mapping the transparent brain with iDISCO+, ClearMap, and light sheet imaging

Sponsored by: Miltenyi Biotec
C.E. Credits: P.A.C.E. CE Florida CE
  • Group Leader, Paris Brain Institute, Paris, France
      After obtaining his master's degree at the Ecole Normale Supérieure, Paris, Nicolas Renier trained in developmental biology in the lab of Alain Chédotal at the Vision Institute in Paris. His work focused on the developmental plasticity of commissural projections, using mouse genetics. For his post-doc, he went to the lab of Marc Tessier-Lavigne at the Rockefeller University in New York. There he co-developed the iDISCO and ClearMap platform for the labeling, imaging, and analysis of intact biological samples using light sheet microscopy and tissue clearing. He established his own lab at the Paris Brain Institute in January 2017.


    DATE:  July 21, 2020

    TIME:   8:00am PDT, 11:00am EDT, 5:00pm CEST


    The adult brain is composed of densely interwoven neuronal and vascular networks that are essential for proper function. Understanding the structural organization and dynamic changes of these networks is highly challenging  because of their complex 3D topology. Dr. Nicolas Renier and his colleagues developed the iDISCO+ and ClearMap technologies to facilitate the study of the structure and cellular networks in the intact adult brain with automated quantitative methods. Together with light sheet fluorescence microscopy (LSFM), these technologies enable whole brain 3D imaging and detailed quantitative mapping. iDISCO+ is a complete sample preparation method that enables whole-mount immunolabeling and high-performance optical clearing of adult brain or any other organ. ClearMap is an open-source software program for the unsupervised mapping of neuronal activity, neuronal projections, and the vascular networks of the brain. These technologies form a pipeline for high-speed acquisition of brain structure and activity at cellular resolution. The workflow consists of sample preparation with immunostaining and tissue clearing, then imaging the whole brain with LSFM for expression profiling, and finally automated mapping and analysis. Dr. Renier will present an overview of these tools and discuss how his team is using them to characterize the plasticity of adult brain networks. 


    Learning Objectives:

    • Learn how to visualize the whole brain in 3D.
    • Discover tools that enable detailed quantitative mapping of complex 3D networks in the brain.
    • Understand the structural organization and dynamic changes of neuronal and vascular networks.



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