APR 17, 2018 8:00 AM PDT

StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based Neural Spheroid Platform for High Throughput Drug Screening

Sponsored by: Molecular Devices
  • Lead Scientist Neurosciences at Stemonix
      Cassiano Carromeu is the Lead Neuroscientist at StemoniX and an expert in the neuroscience and stem cell biology field. He received his BA in Biomedical Sciences and PhD in Virology from the Universidade Federal de Sao Paulo in Brazil. In 2009, he moved to the USA where he did his post-doctoral training at UC San Diego, using induced Pluripotent Stem Cells (iPSCs) to model autism in vitro. During this time, he helped to unveil some key aspects of Rett Syndrome and MeCP2 Duplication Syndrome. He joined StemoniX in 2016, where he has translated his expertise into products with the goal of facilitating drug discovery in neuroscience. His efforts led to the current microBrain products offered by StemoniX.
    • Senior Product Manager, Cellular Imaging at Molecular Devices, LLC.
        Grischa Chandy is the Senior Product Manager for Cellular Imaging at Molecular Devices. Grischa's scientific background is in cell biology, physiology and biophysics. He has used and developed imaging techniques and technologies throughout his career starting with his doctoral work at U.C. Irvine, his postdoc at U.C. Berkeley as well as Research Scientist position at Stanford University. Grischa has been with Molecular Devices for over 10 years, driving launches of the ImageXpress® Micro XL and ImageXpress Micro XLS systems, MetaXpress® Software version 3 onward, and most recently, the launch of the ImageXpress Micro Confocal system.
      • Senior Research Scientist, Molecular Devices
          Oksana Sirenko earned her PhD in Biochemistry from the Biochemistry Research Institute, Academy of Sciences in Ukraine. For over 15 years, she has worked as a Research Scientist in cell-based assay development, drug discovery, and drug development in several pharma and biotech companies including Novartis, Bayer, and Fibrogen. At Molecular Devices she is focused on assay development using high-content imaging systems and other instruments to develop methods for cell-based disease modeling and toxicity research.


        DATE: April 17, 2018
        TIME: 8:00AM PST, 11:00AM EST

        Recently, much research has focused on obtaining 3D brain organoids to better recapitulate brain development and function in vitro. However, current protocols lead to variable organoid size and function, making the use of these powerful tools impractical in drug screening scenarios.

        Here, we describe StemoniX® microBrain® 3D Assay Ready Plates, highly homogenous human induced Pluripotent Stem Cell (hiPSC)-derived cortical spheroid screening platform in a 384-well format. Each well contains a single uniformly sized spheroid, with a fully developed foundation of cortical neurons and astrocytes co-matured from a single donor source. Immunofluorescence analysis indicates that the neurons and astrocytes display key markers of cellular identity and maturity, such as synaptic proteins and glutamate transporters.

        microBrain cortical spheroids present highly synchronized, quantifiable, robust, and uniform spontaneous calcium oscillations, which can be measured in kinetic high throughput screening (HTS) plate readers, such as the FLIPR Tetra® High-Throughput Cellular Screening System, and, at the cellular level, with high content imaging systems, such as the ImageXpress® Micro Confocal High-Content Imaging System. The calcium signal can be reproducibly modified with excitatory and inhibitory neuromodulators, confirming the presence of a functionally integrated glutamatergic and GABAergic circuitry. For HTS applications, microBrain 3D Assay Ready generates remarkably consistent results, displaying minimal variability across wells and plate replicates. Additionally, these cortical organoids are amenable to immunostaining in suspension, enabling scalable high content image-based assays focused on key protein markers. Delivered to customers pre-plated under ambient conditions, this powerful product can be easily implemented as a reliable HTS platform for drug discovery, safety pharmacology and neurotoxicology, and disease modeling.

        Learning Objectives:
        •    Key characteristics of microBrain 3D Assay Ready spheroids
        •    Applications of microBrain 3D Assay Ready for high throughput drug screening
        •    microBrain 3D Assay Ready is delivered to the customer ready to use, integrating easily into their high throughput           screening platforms

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