NOV 16, 2020 8:00 AM PST

Targeted single-cell RNA sequencing enables genome-scale CRISPR screens

Sponsored by: Illumina
Speakers

Abstract
Date:  November 16, 2020
Time: 8:00am (PST),  11:00am (EST),  4:00pm (GMT),  5:00pm (CET)
 
CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screens conducted today rely on rather simplistic read-outs.
 
At Aelian, we use the CROP-seq (CRISPR droplet sequencing) workflow developed by Aelian co-founder Christoph Bock to combine CRISPR screening with single-cell transcriptomics, leveraging two transformative technologies to enable genetic screening for complex phenotypes. Our powerful approach has broad applications in identifying novel drug targets or elucidating unknown mechanisms of actions of drugs.
 
Currently, most single-cell RNA sequencing platforms utilize an unbiased sequencing approach. While powerful, it typically delivers shallow sequencing data for each single cell. At Aelian, we combine CRISPR perturbation with targeted single-cell RNA sequencing using the BD Rhapsody workflow, coupled with Illumina sequencing readout, to focus sequencing resources on the most informative transcripts. The data we gathered suggest that targeted sequencing leads to a reduction in cost as cells can be sequenced at 10x reduced depth. At the same time, the signal-to-noise ratio improves significantly, thus enhancing the dynamic range of CROP-seq screens.
 
Overall, advances presented here will pave the way towards genome-scale single-cell CRISPR screens.
 
In addition, this webinar will see Dr. Pawel Zajac presenting the latest Illumina news and Dr. Wieland Keilholz introducing the BD Rhapsody single-cell analysis system.
 
Learning Objectives
  • Demonstrate the potential of single-cell RNA sequencing as universal read-out for CRISPR screens.
  • Benefits and drawbacks of targeted single-cell sequencing over unbiased whole transcriptome amplification.
  • Introduce the BD Rhapsody workflow, with Illumina sequencing readout, focusing on the high capture rate and cell load.
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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