Revvity's Dharmacon All-in-one lentiviral platform has expanded to include whole-genome library options for CRISPR knockout (CRISPRko), CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa). Like the individual All-in-one reagents, the whole-genome libraries utilize a single vector lentiviral system with all components necessary for CRISPRko, CRISPRa, and CRISPRi whole-genome pooled screening.
Benefits of the Revvity Dharmacon All-in-One CRISPR Platform
- No need to create stable Cas9 expressing cell lines, providing significant time savings of 10 to 30 days
- Ability to target tens of thousands of genes with simple setup (no automation required)
- Highly compatible format for difficult-to-transfect or primary cells that are usually not tolerant of multiple rounds of selection
Avoiding Plasmid Library Challenges
Revvity's All-in-one CRISPR lentiviral libraries eliminate common workflow complications:
- Avoid Library Amplification: No risk of amplification bias affecting sgRNA representation or PCR cycle concerns
- No Colony Growth Delays: Eliminates waiting periods and intercolony competition that can introduce bias
- Skip NGS Confirmation: No need to confirm sgRNA sequence representation before screening
- Eliminate Contamination Steps: No viral contamination concerns or endotoxin-free plasmid purification requirements
- No Viral Packaging: Eliminates tedious packaging procedures and antibiotic selection optimization
- Skip Transfection Optimization: No need to determine optimal cell density for transduction efficacy
All-in-one CRISPR lentiviral vectors for CRISPR knockout, CRISPR activation, and CRISPR interference
Ensuring confidence in screening results with tight distribution and proper representation
High representation of single guide RNAs (sgRNAs) in lentiviral pooled screening is vital for several reasons:
- Guarantees diverse gene coverage, crucial for accurately identifying genes involved in specific biological processes
- Minimizes sampling bias, leading to more reliable results and high-confidence conclusions about gene functions
- Prevents outgrowth of dominant clones, maintaining sgRNA library diversity and validity of findings
Successful screening requires starting with a high-quality library with adequate sgRNA representation to ensure unbiased, even results.
Learn more about the Dharmacon All-in-one CRISPR platform on our applications page or view our related webinar available On-demand now.