Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Thermo Fisher Scientific includes powerful assay design algorithms, optimized master mixes, intuitive data analysis software, and diverse applications. The analytical validation (AV) of qPCR assays can, however, be a complex and time-consuming task.

In this webinar, we will explore the need for AV and dive into what it takes to complete a qPCR AV. An experienced AV Project Manager, Victoria Quiett, will share her insight into the AV journey, focusing on the most accepted validation guidelines, as well as challenges and solutions for a successful AV.

Learning objectives:

• Outline the principal validation guidelines

• Discuss the challenges that come with an AV

• Discover the way Thermo Fisher Scientific consults on an AV project