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SEP 21, 2022 9:00 AM PDT

Arctis Cryo-Plasma-FIB for throughput and connectivity in cellular cryo-electron tomography

Speakers

Abstract
Date:  September 21, 2022
Time: 9:00am (PDT),  12:00pm (EDT), 6:00pm (CEST)
 
Cellular cryo-electron tomography is a high-resolution technique that enables imaging of the molecular machinery of a cell at close-to-native conditions. With the advent of dedicated cryo-FIB instruments, modern energy filters, fast direct electron detectors and advanced data acquisition, exciting new opportunities have opened for cryo-electron tomography to shed light on fundamental questions in cell and structural biology. In this webinar we will present our next generation Arctis Cryo-PFIB microscope pioneering the systematic production of lamellae from cellular samples for cryo-electron tomography. The new microscope features a state-of-the-art plasma ion source, an automatic sample loading system, integrated fluorescence microscopy for correlative microscopy and direct connectivity to the Cryo-TEM. This enables the production of cryo-lamellae with higher throughput and better productivity than ever before.
 
Learning Objectives
  • Discover how cryo-electron tomography enables insights into intact cellular environments.
  • Discuss and explain how the Arctis cryo-plasma FIB works
  • Uncover how Arctis is used for sample preparation in cryo-electron tomography
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

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