The ability of light sheet microscopy to interrogate organoids, whole brain, and systems in 3D enables the study of complex neurological pathways, vasculature and disease models in unique ways. We will discuss considerations in tissue clearing and staining as well as state of the art technology that provides automation, cutting-edge optics, and flexibility of samples from batch imaging all the way up to an entire cleared adult mouse. We will show examples of how this technology is used in several areas of research to study neurological disease and function.

Learning Objectives:

1. Discuss advantages of light sheet fluorescence microscopy.

2. Break down automated high resolution 3D light sheet technology.

3. List practical applications for 3D light sheet microscopy in neuroscience.