MAY 10, 2017 4:00 PM PDT

Ensuring successful protein expression through proper plasmid purification

  • Senior Staff Scientist, Cell Biology, Life Sciences Solutions Group, Thermo Fisher Scientific
      Alexander "Sasha" Vlassov is a Senior Manager, Research and Development, at the Austin site of Thermo Fisher Scientific. Dr. Vlassov has developed multiple product lines including reagents for exosome and microbiome research, siRNA, tools for miRNA functional analysis, and transfection reagents. For the last two years his team is developing advanced technologies for plasmid DNA purification, with primary downstream application being ExpiCHO and Expi293 transient expression systems.


    Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and conditions are chosen to ensure optimal performance. Listen in as our R&D expert discusses the basics and commonly overlooked aspects of plasmid purification to help ensure successful protein expression experiments. In addition, he will review the latest endotoxin-free plasmid purification technology developments at Thermo Fisher Scientific, which includes the PureLinkTM Expi Endotoxin-free plasmid purification portfolio. These kits purify high quality, endotoxin-free plasmid DNA in about half the time, enabling peace-of-mind when going from plasmid to protein.

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