Since 1993, Gibco™ B-27™ Supplement and Neurobasal™ Medium has been the trusted standard for a variety of neuronal culture applications, with citations in more than 11,000 publications. However, as the desire for more reliable and biologically relevant models has increased, so too has the necessity for a media system that can maintain and mature functional neurons over long periods of time in culture. To meet this need, we have developed the new Gibco™ B-27™ Plus Neuronal Culture System comprising a neuronal basal medium (Neurobasal™ Plus) and serum-free supplement (B-27™ Plus) that provides significant improvements for long term viability and functionality of primary and PSC-derived neurons in vitro.
This next-generation media system features an optimized formulation, upgraded manufacturing process, and more stringent quality control for raw materials and final product.
Performance of this system was evaluated by a number of criteria including neurite outgrowth, neuronal survival and functionality in primary rat, mouse and human PSC-derived neurons for long term cultures at both low and high cell density. Taken together the results demonstrate that our new B-27™ Plus Neuronal Culture System is a superior solution to the current trusted standards used for culturing primary neurons, and maturing and maintaining hPSC-derived neurons.