The Neon transfection system enables robust transfection efficiencies in hiPSC for DNA, mRNA and Cas9/gRNA RNP complexes. For hiPSC these improved transfection as well as post-electroporation culture conditions are essential for increased cell survival for downstream applications. This live training will review protocols involving Neon electroporation of hiPSC and post electroporation culture, using delivery of CRISPR/Cas9-mediated genome editing tools as the example.