OCT 12, 2023 9:00 AM PDT

Mitigating the effects of spillover/spread in multicolor panels.

Sponsored by: BD
Speaker

Event Date & Time
Date: October 12, 2023
Time: 9:00 AM (PDT), 12:00 PM (EDT), 6:00 PM (CEST)
Abstract
Multicolor flow cytometry is beneficial because it allows us to gain deeper insights from a given biological sample, with fewer repeat markers in each tube and quicker results. In this three-part webinar series, we will identify the fundamental principles of panel design. In the first webinar, we showed how sample biology can inform fluorochromes choice. In this webinar we will focus on the effects of spillover/spread on data resolution and how to overcome them. And in the next webinar, we will focus on the instrument-related factors that can influence panel design. Join us for all three webinars to understand the principles of panel design and how to use them to troubleshoot panels in your lab.
 
Learning Objectives
  • Identify the main sources of spillover in your panel
  • Recognize the utility of proper compensation controls
  • Apply panel design rules to mitigate spread in multicolor panels
 
 
Sign up for the other webinars in this series on September 26th and November 15th: 
 
Flow cytometry enables simultaneous analysis of multiple markers at the single-cell level. With the increase in number of detectable parameters, the design of a multicolor panel can be challenging and requires an understanding of several factors that can influence panel performance. This three-part webinar series will cover the basic pillars of panel design and standardization:
 
Biology—how antigen density and co-expression can affect fluorochrome choice
Fluorochrome—how spillover spread can be predicted and mitigated
Instrument—how instrument standardization can influence data resolution
 
Make sure to join all three talks in the series to learn how you can troubleshoot existing panels in your clinical flow cytometry lab.
 
 
Webinars will be available for unlimited on-demand viewing after live event.

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