MENU
MAY 10, 2017 3:00 PM PDT

Large scale serum free suspension lentiviral production for cell and gene therapy application

Speaker
  • Scientist II, Cell Biology, Life Sciences Solutions Group, Thermo Fisher Scientific
    Biography
      Yongchang Ji, PhD. is a scientist in the Life Sciences Solutions Group at Thermo Fisher Scientific in Carlsbad, CA. Yongchang is currently working on development of the high tier, large scale mammalian suspension lentiviral vector production system for gene therapy application. Dr. Ji received his Ph.D. in Cell Biology and Anatomy from SUNY Upstate Medical University, NY and his undergraduate degree from Xiamen University, China.

    Abstract

    Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade production of Lentiviral vectors on a large-scale serum-free suspension platform. This technology employs a newly developed propriety set of GMP reagents comprising of culture media, suspension cells, transfection reagent and boosting enhancers. With new culture media and media supplement, high density cell growth is optimized for maximum LVVs production. Customized lentivirus transfection reagents ensure highly efficient plasmids delivery. LVVs production is further elevated by the boosting enhancers. Taken together, our innovative system is able to deliver >2-3.0E+08 (TU/mL) functional titer with un-concentrated LVVs. We demonstrate that this system can be linearly adapted to bioreactors and wavebags system for large scale LVVs production without compromising the virus yield, which is ideal for industrial needs of gene therapy.


    Show Resources
    MAY 10, 2017 3:00 PM PDT

    Large scale serum free suspension lentiviral production for cell and gene therapy application


    Specialty

    Genetics

    Biotechnology

    Cell Biology

    Molecular Biology

    Virology

    Cell Culture

    Cancer Research

    Cancer Diagnostics

    Medicine

    Immunology

    Microbiology

    Oncology

    Pcr/rt-Pcr/real-Time Pcr

    Laboratory Testing

    Drug Discovery

    Geography

    North America78%

    Asia13%

    Europe5%

    South America3%

    Africa3%

    Registration Source

    Website Visitors100%

    Job Title

    Facility/Department Manager16%

    Scientist16%

    Student14%

    Executive14%

    Educator/Faculty14%

    Research Scientist11%

    Medical Doctor/Specialist8%

    Microbiologist3%

    Genetic Counselor3%

    Lab Management3%

    Organization

    Academic Institution15%

    Biotech Company10%

    Life Science Company8%

    Clinical Laboratory5%

    Manufacturer & Biotech/pharma3%

    Government3%

    Ambulatory Care3%

    Consultant3%

    Media3%

    Industrial Company3%

    Manufacturer - Other3%

    Medical School3%

    Research Institute3%

    Other8%


    Show Resources
    Show Resources
    Attendees
    • See more
    Connect with us