JUN 09, 2020 3:00 PM CEST

Smart Plastics: Improving qPCR reliability & reproducibility

Sponsored by: Eppendorf
C.E. Credits: P.A.C.E. CE Florida CE
  • Professor of Molecular Physiology at the Technical University of Munich
      ​Michael W. Pfaffl started 1986 to study 'Agriculture - Animal Science' and 'Biotechnology' at the Technical University of Munich (TUM). In 1997 he obtained his PhD in 'Molecular Physiology' in the field of molecular muscle and growth physiology at the Chair of Physiology. In June 2003 he completed his Venia Legendi (Dr. habil.) at the Center of Life and Food Sciences Weihenstephan with the title 'Livestock transcriptomics -- Quantitative mRNA analytics in molecular endocrinology and mammary gland physiology'.

      ​Early 2010 he became Professor of 'Molecular Physiology' at the TUM School of Life Sciences. Today he has reached the 'Principal Investigator' status at the Institute of Animal Physiology & Immunology and is one of the leading scientists in the field of Gene Quantification, RT-qPCR technology, RNA sequencing, and complex data analysis in mRNA & small-RNA expression profiling.
      He is author of around 180 peer reviewed publications, 45 book chapters, and held more than 230 lectures worldwide. In March 2012 the Elsevier SciVerse Scopus Award 2012 was granted to Prof. Michael W. Pfaffl, whose top cited Scopus article entitled "A new mathematical model for relative quantification in real-time RT-PCR" published 2001 in Nucleic Acids Research 29(9) has been cited today more than 21,800 times. He is coauthor of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines (2009) and coauthor of the dMIQE guidelines for digital PCR (2013).

      Professor Michael W. Pfaffl has editorial involvements as Founding- & Section-Editor in 'Biomolecular Detection and Quantification', Editor in 'Methods' and 'International Journal of Oncology', and Editor-in-Chief of the 'Gene Quantification' webportal, the world biggest webpage around qPCR, dPCR and Gene Expression profiling techniques and applications. He is initiator and lead organizer of the qPCR, dPCR & NGS Gene Quantification Event series in Freising Weihenstephan in Germany since 2004 (www.eConferences.de).
    • Global Marketing Manager, Automation and PCR, Eppendorf AG
        Dr. Tim Schommartz works as Application Specialist for automated Liquid Handling at the Eppendorf Headquarter in Hamburg, Germany. He studied Molecular Life Science in Germany and Singapore, focusing on the influence of anti-inflammatory antibodies on allergies and autoimmune diseases during his master thesis. Tim Schommartz holds a PhD in Chemistry working in the field of Virology. He joined Eppendorf in 2016 having a broad background in different workflows, ranging from molecular biology, cell- and infection biology to immunology and biochemistry.
      • Field Application Specialist, Eppendorf AG
          Dr. Rafal Grzeskowiak is a Field Application Specialist responsible for the consumables product portfolio at Eppendorf AG Headquarter in Hamburg (Germany). He joined the company in 2003 and worked at various positions in sales, customer support and marketing with product responsibility for automation, centrifugation and consumables. Rafal obtained his M.Sc. in molecular biology at the University of Warsaw (Poland) working on metabolic gene expression through RNA aptamers in Aspergillus and as a research assistant at King's College London was involved in projects on cloning and phylogenetic analysis of rRNA genes in pathogenic fungi. Rafal received his Ph.D. at the Georg August Universität Göttingen, where he investigated regulation of gene expression and cellular signaling pathways involved in Diabetes Type II. Later he took a position of a postdoctoral research fellow at the Max-Planck Institute for Molecular genetics (Berlin, Germany) and led projects on functional genomics of human dilated cardiomyopathy.

        DATE:  June 9, 2020
        TIME:   6am PT, 9am ET, 3pm CEST
        The importance of disposable plastic consumables and their overall impact on the experimental workflow of qPCR has been taken into consideration only marginally, if at all. This is unfortunate, since laboratory plastics, such as tubes, tips and plates, are integral parts of any laboratory and qPCR workflow. Contrary to common belief, plastics may have a direct and dramatic impact on experimental outcome and data validity.
        Join live for a Q&A session with experts!
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        If you can´t make it, just register to receive the link to the recorded session.
        In this webinar you will learn:
        • What are MIQE Guidelines and their relevance for improving qPCR reproducibility
        • Smart consumables -  how they may contribute to improve experimental reproducibility and data quality
        • LoBind, Leaching, PCR Consumables
        Webinars will be available for unlimited on-demand viewing after live event.
        LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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