From drug discovery to organoid modeling of disease, stem cells are increasingly being used in research as a vital tool for scientific investigation.

Due to the specialized nature of stem cells, their maintenance and culture is more intensive than most cell lines. For this reason, it is important that reliable solutions for the culture and maintenance of these cell types are readily and widely available.

Key webinar discussion topics include:

• How to pick specific iPSC colonies consistently and reliably for further testing or culture while maintaining high levels of cell health
• How to monitor delicate iPSC line culture morphology and growth characteristics without removing plates from the incubator
• The skills needed to use minimal sample volumes required to characterize precious cell types, with minimal attrition for downstream requirements

The study of 3D organoids has transformed biomedical research, offering a more accurate representation of in vivo conditions. Maximizing the potential of 3D organoid research requires advanced techniques that can standardize 3D structure transfer while preserving their viability and morphology during the process. Isolation techniques enable the study of individual cells within the organoid, revealing cellular heterogeneity and interactions. 
 
High-throughput 3D structure transfer for further characterization accelerates our understanding of organoid biology, facilitates the development of more effective treatments for various diseases and paves the way for personalized medicine, as organoids can be generated from patient-derived cells. However, technical challenges remain, necessitating further research and technological advancements. Join this presentation to learn more about the benefits and challenges of using automated isolation technology for 3D organoid research, highlighting their significant contributions to the field.

Organoid technologies are increasingly being used as in vitro models of human development and disease because they exhibit morphogenetic, structural and functional properties that recapitulate in vivo pathophysiology. To successfully use these models across a variety of research disciplines and applications, it is important to reduce image variability, and to quantify these complex cell models.

Key webinar discussion topics include:

• Creation of an integrated turnkey solution, to automatically monitor and quantify, stem cell-derived organoid formation, health, growth and modulation in real-time, from inside your tissue culture incubator
• Incucyte® Live-Cell Analysis System methods, to optimize organoid culture conditions, together with real-time kinetic imaging and quantification, of organoid growth and maturation in Matrigel® domes
• The study and quantification of drug-induced cellular changes in organoids, embedded in Matrigel® domes, using 96-well plates