SEP 17, 2020 11:00 AM EDT

Keynote Presentation: Adding Dimensions to Intravital Imaging

  • Professor of Biology, Bioengineering, & Convergent Biosciences University of Southern California
      Professor Scott E. Fraser has a long-standing commitment to quantitative biology, applying the tools of chemistry, engineering, and physics to problems in biology and medicine. His personal research centers on imaging and molecular analyses of intact biological systems, with an emphasis on early development, organogenesis, and medical diagnostics.

      After training in physics (B.S., Harvey Mudd College, 1976) and biophysics (Ph.D., Johns Hopkins University, 1979), he joined the faculty at UC Irvine, and rose through the ranks to become Chair of the Department of Physiology and Biophysics. In 1990 he moved to Caltech to serve as the Anna L. Rosen Professor of Biology, and the Director of the Biological Imaging Center. He is deeply committed to interdisciplinary training and translational research, having helped found the Caltech Brain Imaging Center and the Kavli Institute of Nanoscience, as well as serving as the Director of the Rosen Center for Biological Engineering.

      In Fall 2012, he moved to USC to take a Provost Professorship in the Dornsife College of Letters Arts and Sciences, the Children's Hospital Los Angeles, Keck School of Medicine and the Viterbi School of Engineering. He remains active in interdisciplinary research and serves as the Director of Science Initiatives for the USC campuses.


    Imaging offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of embryonic development; however, it is challenged by tradeoffs between speed, resolution, field of view and the photon budget. We are advancing this tradeoff by constructing two-photon light-sheet microscopes, combining the deep penetration of two-photon microscopy and the speed of light sheet microscopy, permitting 4D cell and molecular imaging with sufficient speed and resolution to generate unambiguous tracing of cells and signals in intact systems. To increase the 5 th Dimensions, we are refining a new generation of multispectral image analysis tools that exceed the performance of our previous work on Linear Unmixing by orders of magnitude in speed, error propagation and accuracy. These new analysis tools permit rapid and unambiguous analyses of multiplex-labeled specimens. Finally, to move to faster volumetric imaging, we have combined light field and light sheet approaches, offering the signal to noise needed to image thousands of neurons with high fidelity. Combined, these tools offer the multi-dimensional imaging required to follow key events in as they take place and allow us to use variance as an experimental tool rather than feeling its effects as a limitation.

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