SEP 16, 2020 2:00 PM EDT

Mapping Tissues with In Situ Single-Cell Transcriptomics

  • Boston Children's Hospital
      Dr. Jeffrey Moffitt received his PhD in Physics from the University of California Berkeley under the direction of Dr. Carlos Bustamante, where he developed novel optical manipulation methods to study molecular motors. He received postdoctoral training from Dr. Xiaowei Zhuang at Harvard University, where he developed methods for transcriptome-scale imaging in single cells. In particular, he was a co-inventor of the spatially resolved single-cell transcriptomics approach known as MERFISH. Dr. Moffitt is an Investigator in the Program in Cellular and Molecular Medicine at Boston Children's Hospital and an Assistant Professor in the Department of Microbiology at Harvard Medical School. His laboratory is developing new spatially resolved methods for single-cell 'omics and using these tools to discover and map cells in a wide range of tissues. His laboratory is supported by a Pew Biomedical Scholar Award, the NIH, and the Chan Zuckerberg Initiative.


    Image-based approaches to single-cell transcriptomics are emerging as powerful complements to single-cell RNA sequencing, in part, because these techniques preserve the native spatial context of RNAs within cells and tissues. I will describe multiplexed error robust single-molecule fluorescence in situ hybridization (MERFISH)—a technique capable of imaging thousands of different RNAs simultaneously in fixed cells—and its use for the discovery and mapping of cell types within intact tissues.

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