This product provides special reaction conditions to better support the formation of disulfide bonds in proteins expressed in the wheat germ cell-free expression system. Reaction conditions have been revised by adding a human endoplasmic reticulum oxidoreductase (Ero1α) and a Protein disulfide isomerase (PDI) to the translation reaction while using a different buffer composition for bilayer formation. Learn more here on how to we support disulfide bond formation in our expression system./

※ Before using this product, confirm whether your target protein requires disulfide bond formation for correct folding and activity. Do not use this product when working with the GST-tag for later purification. Disulfide bonds are not stable when using glutathione containing buffers during the elution step./

Features:

• Reagents necessary for each reaction are already premixed (Ero1a and PDI mixture provided separately)

• Synthesizing proteins with just a few pipetting steps

• Target proteins can be confirmed through SDS-PAGE/CBB staining

• Kit comes with expression vector, and primers to support template preparation

• Kit provided positive control to confirm formation of disulfide bonds/

Recommended for:

• Users who want to prepare proteins having disulfide bonds

• Users who have difficulties with a protein of interest can try these reaction conditions when no prior information on disulfide bond formation is available to test better solubility and activity

• Users who want to test reaction conditions for scaling up protein expression/

The basic reagents necessary for each reaction step are pre-packaged into tubes, so there is no need to prepare any reagents.

Enzyme mix is provided separately.