This kit was designed to be user-friendly even for first-time users.

Our wheat germ cell-free expression system allows you to easily make full-length isotope-labeled MS standards.

Features:

• Incorporates stable 13C and 15N isotopes-labeled lysine and arginine residues suitable for working with trypsin digestion in bottom-up proteomics

• Very high labeling efficiency with incorporation rates of over 99%

• No need to purchase 13C and 15N stable isotope-labeled amino acids as they are already added to translation system

• Special expression and control vector provided to utilize FLEX-tag for absolute protein quantification by FLEXIQuant method

• All reagents included to directly prepare labeled proteins from DNA template

• Premixed reagents to synthesize proteins with just a few pipetting steps

• Translation buffer provided in special reaction cups for bilayer reactions

• Target protein expression can be confirmed through SDS-PAGE/CBB staining

• Kit contains reagents for 8 translation reactions on 226 µl reaction scale

Recommended for:

• Users who require full-length isotope-labeled MS standards easily using wheat germ cell-free expression system/

All reagents are already aliquoted to provide the amounts needed per reaction.

This makes reaction setup easy and avoids unnecessary freeze-thawing of sensitive reagents.

Simply cut off the number of tubes needed for each experiment.



*1 : WEPRO9240 for MS is based on the WEPRO8240 with the addition of 13C and 15N-labeled lysine, arginine, and Creatine Kinase.

*2 : SUB-AMIX SGC for MS includes 18 types of non-labeled amino acids excluding lysine and arginine.

*3 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.