MICROBIAL TRANSGLUTAMINASE (MTG) cleaves a dark quenching molecule from the side chain of MTG specific peptide A167 incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored (excitation wavelength 330 nm; emission wavelength 418 nm)./
Reagents in the kit
(1) SUBSTRATE REAGENT (SR): 2 x 23 μL MTG specific peptide A167 (DMSO solution).
(2) BUFFER REAGENT (BR): 2 x 18 mL TRIS buffer pH 6.0 containing glycine methyl ester (lyophilizate).
(3) DILUTION BUFFER (DB): 1 x 18 mL TRIS buffer pH 6.0 (lyophilizate)./
Equipment
The ZediXcite Fluorogenic MTG-Assay Kit (F015) can be used in fluorescence microplate readers using microplates as well as in standard fluorescence spectrophotometers with cuvettes. Refer to the instructions of the manufacturer.